Focusing on the issue that the Low-Rank Representation (LRR) subspace clustering algorithm does not consider the local structure of the data and may cause the loss of local similar information during learning, a Low-Rank Representation subspace clustering algorithm based on Hessian regularization and Non-negative constraint (LRR-HN) was proposed to explore the global and local structure of the data. Firstly, the good speculative ability of Hessian regularization was used to maintain the local manifold structure of the data, so that the local topological structure of the data was more expressive. Secondly, considering that the obtained coefficient matrix often has positive and negative values, and the negative values often have no practical significance, non-negative constraints were introduced to ensure the effectiveness of the model solution and make it more meaningful in the description of the local structure of the data. Finally, the low-rank representation of the global structure of the data was sought by minimizing the nuclear norm, so as to cluster high-dimensional data better. In addition, an effective algorithm for solving LRR-HN was designed by using the linearized alternating direction method with adaptive penalty, and the proposed algorithm was evaluated by ACcuracy (AC) and Normalized Mutual Information (NMI) on some real datasets. In the experiments with clusters number 20 on ORL dataset, compared with LRR algorithm, LRR-HN has the AC and NMI increased by 11% and 9.74% respectively, and compared with Adaptive Low-Rank Representation (ALRR) algorithm, LRR-HN has the AC and NMI increased by 5% and 1.05% respectively. Experimental results show that the LRR-HN has great improvement in AC and NMI compared with some existing algorithms, and has the excellent clustering performance.